dapi fluorescent stain Search Results


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Edmund Optics fluorescent microscope with filters to visualize either dapi or pi stain
Fluorescent Microscope With Filters To Visualize Either Dapi Or Pi Stain, supplied by Edmund Optics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Applichem inc dna-binding fluorescent stain 4',6-diamidino-2-phenylindole
Dna Binding Fluorescent Stain 4',6 Diamidino 2 Phenylindole, supplied by Applichem inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss 4′,6-diamidino-2-phenylindole (dapi) fluorescent stain cell nuclei
4′,6 Diamidino 2 Phenylindole (Dapi) Fluorescent Stain Cell Nuclei, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Servicebio Inc fluorescent nuclear staining reagent dapi
Fluorescent Nuclear Staining Reagent Dapi, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biozol Diagnostica Vertrieb GmbH dapi fluorescent stain
Dapi Fluorescent Stain, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Partec fluorescent dna stain 4, 6-diamidino-2-phenylindole (dapi)
Fluorescent Dna Stain 4, 6 Diamidino 2 Phenylindole (Dapi), supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescent dna stain 4, 6-diamidino-2-phenylindole (dapi)/product/Partec
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Matisse Pharmaceuticals fluorescent microscopy images based on dapi nuclear staining
Combining fluorescence microscopy with multiplex IMC data of colorectal tissue advances quality of single cell segmentation. a Cartoon describing MATISSE, a novel pipeline adding microscopic imaging to multiplex IMC analysis and downstream segmentation. In short: tissue sections on slides were stained using isotope-conjugated primary antibodies, DNA intercalator, and <t>DAPI.</t> The tissue was first scanned using <t>a</t> <t>fluorescent</t> microscope and then processed with IMC. Data produced by both techniques is aligned using the nuclear staining. Nuclear and membranous pixel probability maps are produced based on the fluorescent images and IMC data respectively. These probability maps are used to generate a segmentation map, where all detected cells are included. b Representative images of DNA intercalator on a colorectal tissue section analyzed by Ir193 labeling and IMC (left) or DAPI labeling and fluorescent microscopy (IF, right). c IMC-only (IMC) and MATISSE cell segmentation (MATISSE) were performed, and shown are the different predicted outlines on a representative image of Ir193 labeling. Arrows indicate areas with cell fragmentation. d Display of a large region of interest (ROI) showing an overlay of the predicted cell outlines (pink) upon IMC or MATISSE segmentation on a representative IMC image of DNA-Ir193 labeling of colorectal tissue. Highlighted in yellow is the approximate position of the basement membrane surrounding the epithelial monolayer. Scale bar 25 μm. e Cell density was calculated as the number of cells within a radius of 10 μM from the center of each single cell [ , ]. This number is displayed with a color code for each cell in the representative image
Fluorescent Microscopy Images Based On Dapi Nuclear Staining, supplied by Matisse Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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LabClinics SA fluorescence microscope dapi stain
Combining fluorescence microscopy with multiplex IMC data of colorectal tissue advances quality of single cell segmentation. a Cartoon describing MATISSE, a novel pipeline adding microscopic imaging to multiplex IMC analysis and downstream segmentation. In short: tissue sections on slides were stained using isotope-conjugated primary antibodies, DNA intercalator, and <t>DAPI.</t> The tissue was first scanned using <t>a</t> <t>fluorescent</t> microscope and then processed with IMC. Data produced by both techniques is aligned using the nuclear staining. Nuclear and membranous pixel probability maps are produced based on the fluorescent images and IMC data respectively. These probability maps are used to generate a segmentation map, where all detected cells are included. b Representative images of DNA intercalator on a colorectal tissue section analyzed by Ir193 labeling and IMC (left) or DAPI labeling and fluorescent microscopy (IF, right). c IMC-only (IMC) and MATISSE cell segmentation (MATISSE) were performed, and shown are the different predicted outlines on a representative image of Ir193 labeling. Arrows indicate areas with cell fragmentation. d Display of a large region of interest (ROI) showing an overlay of the predicted cell outlines (pink) upon IMC or MATISSE segmentation on a representative IMC image of DNA-Ir193 labeling of colorectal tissue. Highlighted in yellow is the approximate position of the basement membrane surrounding the epithelial monolayer. Scale bar 25 μm. e Cell density was calculated as the number of cells within a radius of 10 μM from the center of each single cell [ , ]. This number is displayed with a color code for each cell in the representative image
Fluorescence Microscope Dapi Stain, supplied by LabClinics SA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescence microscope dapi stain/product/LabClinics SA
Average 90 stars, based on 1 article reviews
fluorescence microscope dapi stain - by Bioz Stars, 2026-03
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Merck KGaA fluorescent dna stain dapi
Combining fluorescence microscopy with multiplex IMC data of colorectal tissue advances quality of single cell segmentation. a Cartoon describing MATISSE, a novel pipeline adding microscopic imaging to multiplex IMC analysis and downstream segmentation. In short: tissue sections on slides were stained using isotope-conjugated primary antibodies, DNA intercalator, and <t>DAPI.</t> The tissue was first scanned using <t>a</t> <t>fluorescent</t> microscope and then processed with IMC. Data produced by both techniques is aligned using the nuclear staining. Nuclear and membranous pixel probability maps are produced based on the fluorescent images and IMC data respectively. These probability maps are used to generate a segmentation map, where all detected cells are included. b Representative images of DNA intercalator on a colorectal tissue section analyzed by Ir193 labeling and IMC (left) or DAPI labeling and fluorescent microscopy (IF, right). c IMC-only (IMC) and MATISSE cell segmentation (MATISSE) were performed, and shown are the different predicted outlines on a representative image of Ir193 labeling. Arrows indicate areas with cell fragmentation. d Display of a large region of interest (ROI) showing an overlay of the predicted cell outlines (pink) upon IMC or MATISSE segmentation on a representative IMC image of DNA-Ir193 labeling of colorectal tissue. Highlighted in yellow is the approximate position of the basement membrane surrounding the epithelial monolayer. Scale bar 25 μm. e Cell density was calculated as the number of cells within a radius of 10 μM from the center of each single cell [ , ]. This number is displayed with a color code for each cell in the representative image
Fluorescent Dna Stain Dapi, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescent dna stain dapi - by Bioz Stars, 2026-03
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Partec flow cytometry histograms based on dapi fluorescence staining
Combining fluorescence microscopy with multiplex IMC data of colorectal tissue advances quality of single cell segmentation. a Cartoon describing MATISSE, a novel pipeline adding microscopic imaging to multiplex IMC analysis and downstream segmentation. In short: tissue sections on slides were stained using isotope-conjugated primary antibodies, DNA intercalator, and <t>DAPI.</t> The tissue was first scanned using <t>a</t> <t>fluorescent</t> microscope and then processed with IMC. Data produced by both techniques is aligned using the nuclear staining. Nuclear and membranous pixel probability maps are produced based on the fluorescent images and IMC data respectively. These probability maps are used to generate a segmentation map, where all detected cells are included. b Representative images of DNA intercalator on a colorectal tissue section analyzed by Ir193 labeling and IMC (left) or DAPI labeling and fluorescent microscopy (IF, right). c IMC-only (IMC) and MATISSE cell segmentation (MATISSE) were performed, and shown are the different predicted outlines on a representative image of Ir193 labeling. Arrows indicate areas with cell fragmentation. d Display of a large region of interest (ROI) showing an overlay of the predicted cell outlines (pink) upon IMC or MATISSE segmentation on a representative IMC image of DNA-Ir193 labeling of colorectal tissue. Highlighted in yellow is the approximate position of the basement membrane surrounding the epithelial monolayer. Scale bar 25 μm. e Cell density was calculated as the number of cells within a radius of 10 μM from the center of each single cell [ , ]. This number is displayed with a color code for each cell in the representative image
Flow Cytometry Histograms Based On Dapi Fluorescence Staining, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beuth Verlag GmbH fluorescence analysis after staining with dapi
Combining fluorescence microscopy with multiplex IMC data of colorectal tissue advances quality of single cell segmentation. a Cartoon describing MATISSE, a novel pipeline adding microscopic imaging to multiplex IMC analysis and downstream segmentation. In short: tissue sections on slides were stained using isotope-conjugated primary antibodies, DNA intercalator, and <t>DAPI.</t> The tissue was first scanned using <t>a</t> <t>fluorescent</t> microscope and then processed with IMC. Data produced by both techniques is aligned using the nuclear staining. Nuclear and membranous pixel probability maps are produced based on the fluorescent images and IMC data respectively. These probability maps are used to generate a segmentation map, where all detected cells are included. b Representative images of DNA intercalator on a colorectal tissue section analyzed by Ir193 labeling and IMC (left) or DAPI labeling and fluorescent microscopy (IF, right). c IMC-only (IMC) and MATISSE cell segmentation (MATISSE) were performed, and shown are the different predicted outlines on a representative image of Ir193 labeling. Arrows indicate areas with cell fragmentation. d Display of a large region of interest (ROI) showing an overlay of the predicted cell outlines (pink) upon IMC or MATISSE segmentation on a representative IMC image of DNA-Ir193 labeling of colorectal tissue. Highlighted in yellow is the approximate position of the basement membrane surrounding the epithelial monolayer. Scale bar 25 μm. e Cell density was calculated as the number of cells within a radius of 10 μM from the center of each single cell [ , ]. This number is displayed with a color code for each cell in the representative image
Fluorescence Analysis After Staining With Dapi, supplied by Beuth Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss dapi staining under zeiss axioskop fluorescence microscope
Combining fluorescence microscopy with multiplex IMC data of colorectal tissue advances quality of single cell segmentation. a Cartoon describing MATISSE, a novel pipeline adding microscopic imaging to multiplex IMC analysis and downstream segmentation. In short: tissue sections on slides were stained using isotope-conjugated primary antibodies, DNA intercalator, and <t>DAPI.</t> The tissue was first scanned using <t>a</t> <t>fluorescent</t> microscope and then processed with IMC. Data produced by both techniques is aligned using the nuclear staining. Nuclear and membranous pixel probability maps are produced based on the fluorescent images and IMC data respectively. These probability maps are used to generate a segmentation map, where all detected cells are included. b Representative images of DNA intercalator on a colorectal tissue section analyzed by Ir193 labeling and IMC (left) or DAPI labeling and fluorescent microscopy (IF, right). c IMC-only (IMC) and MATISSE cell segmentation (MATISSE) were performed, and shown are the different predicted outlines on a representative image of Ir193 labeling. Arrows indicate areas with cell fragmentation. d Display of a large region of interest (ROI) showing an overlay of the predicted cell outlines (pink) upon IMC or MATISSE segmentation on a representative IMC image of DNA-Ir193 labeling of colorectal tissue. Highlighted in yellow is the approximate position of the basement membrane surrounding the epithelial monolayer. Scale bar 25 μm. e Cell density was calculated as the number of cells within a radius of 10 μM from the center of each single cell [ , ]. This number is displayed with a color code for each cell in the representative image
Dapi Staining Under Zeiss Axioskop Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dapi staining under zeiss axioskop fluorescence microscope/product/Carl Zeiss
Average 90 stars, based on 1 article reviews
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Image Search Results


Combining fluorescence microscopy with multiplex IMC data of colorectal tissue advances quality of single cell segmentation. a Cartoon describing MATISSE, a novel pipeline adding microscopic imaging to multiplex IMC analysis and downstream segmentation. In short: tissue sections on slides were stained using isotope-conjugated primary antibodies, DNA intercalator, and DAPI. The tissue was first scanned using a fluorescent microscope and then processed with IMC. Data produced by both techniques is aligned using the nuclear staining. Nuclear and membranous pixel probability maps are produced based on the fluorescent images and IMC data respectively. These probability maps are used to generate a segmentation map, where all detected cells are included. b Representative images of DNA intercalator on a colorectal tissue section analyzed by Ir193 labeling and IMC (left) or DAPI labeling and fluorescent microscopy (IF, right). c IMC-only (IMC) and MATISSE cell segmentation (MATISSE) were performed, and shown are the different predicted outlines on a representative image of Ir193 labeling. Arrows indicate areas with cell fragmentation. d Display of a large region of interest (ROI) showing an overlay of the predicted cell outlines (pink) upon IMC or MATISSE segmentation on a representative IMC image of DNA-Ir193 labeling of colorectal tissue. Highlighted in yellow is the approximate position of the basement membrane surrounding the epithelial monolayer. Scale bar 25 μm. e Cell density was calculated as the number of cells within a radius of 10 μM from the center of each single cell [ , ]. This number is displayed with a color code for each cell in the representative image

Journal: BMC Biology

Article Title: MATISSE: a method for improved single cell segmentation in imaging mass cytometry

doi: 10.1186/s12915-021-01043-y

Figure Lengend Snippet: Combining fluorescence microscopy with multiplex IMC data of colorectal tissue advances quality of single cell segmentation. a Cartoon describing MATISSE, a novel pipeline adding microscopic imaging to multiplex IMC analysis and downstream segmentation. In short: tissue sections on slides were stained using isotope-conjugated primary antibodies, DNA intercalator, and DAPI. The tissue was first scanned using a fluorescent microscope and then processed with IMC. Data produced by both techniques is aligned using the nuclear staining. Nuclear and membranous pixel probability maps are produced based on the fluorescent images and IMC data respectively. These probability maps are used to generate a segmentation map, where all detected cells are included. b Representative images of DNA intercalator on a colorectal tissue section analyzed by Ir193 labeling and IMC (left) or DAPI labeling and fluorescent microscopy (IF, right). c IMC-only (IMC) and MATISSE cell segmentation (MATISSE) were performed, and shown are the different predicted outlines on a representative image of Ir193 labeling. Arrows indicate areas with cell fragmentation. d Display of a large region of interest (ROI) showing an overlay of the predicted cell outlines (pink) upon IMC or MATISSE segmentation on a representative IMC image of DNA-Ir193 labeling of colorectal tissue. Highlighted in yellow is the approximate position of the basement membrane surrounding the epithelial monolayer. Scale bar 25 μm. e Cell density was calculated as the number of cells within a radius of 10 μM from the center of each single cell [ , ]. This number is displayed with a color code for each cell in the representative image

Article Snippet: We observed that incorporating fluorescent microscopy images based on DAPI nuclear staining into MATISSE workflow resulted in superior visual and signal intensity-based separation of nuclei in dense areas (Fig. b, Additional file : Fig S1B).

Techniques: Fluorescence, Microscopy, Multiplex Assay, Imaging, Staining, Produced, Labeling, Membrane